Fig. 3

Higher Tumor-Associated Treg Cell Infiltration in Pancreatic Cancer Samples with High OSBPL3. (A) Expression Proportion of FOXP3 and CD4 co-expressing cells in pancreatic cancer samples and their relative abundance in high (High-OSBPL3) and low (Low-OSBPL3) OSBPL3 expression groups. (B) Proportion of IL2RA and CD4 co-expressing cells in pancreatic cancer samples and their relative abundance in High-OSBPL3 and Low-OSBPL3 groups. (C) Dimensionality reduction and clustering of Treg cell subpopulations. Different colors represent distinct subclusters, and the differential expression genes for each subcluster are displayed. (D) Monocle trajectory analysis of Treg cell subpopulations, illustrating the inferred pseudotime progression across different subclusters. (E) Heatmap showing gene sets and their enriched pathways identified through pseudotime analysis across all Treg cell subpopulations. (F) Bubble plot depicting the expression of Treg cell marker genes across different Treg subpopulations. (G) Expression of FOXP3, LAG3, and OSBPL3 in Treg cells. (H) Proportions of different Treg subpopulations in High-OSBPL3 and Low-OSBPL3 groups. (I) Density plot showing the distribution of OSBPL3 expression across different Treg subpopulations