Fig. 4

Macrophages with High OSBPL3 Expression Exhibit a Stronger M2 Polarization Phenotype. (A) UMAP dimensionality reduction plot showing the distribution of myeloid cell subpopulations in pancreatic cancer samples, with differential marker genes identified for each subpopulation. (B) Bar plot displaying the proportion of CD68 and MRC1 or CD163 co-expressing cells in the high (High-OSBPL3) and low (Low-OSBPL3) OSBPL3 expression groups. (C) Monocle pseudotime analysis showing the trajectory of different cell subpopulations and OSBPL3 expression groups. (D) Heatmap illustrating key genes and enriched pathways identified from gene sets derived through monocle pseudotime analysis. (E) Violin plot comparing M2 macrophage polarization scores between High-OSBPL3 and Low-OSBPL3 groups. (F) Violin plot showing M2 macrophage polarization scores across different cell subpopulations. (G) UMAP plot displaying the proportions of functional subpopulations of myeloid cells and the expression of OSBPL3 within these subpopulations. (H) Scatter plot comparing the differential expression of M2 polarization-related genes between OSBPL3 knockdown and control groups. The x-axis represents Log2FC values for the two groups