Fig. 5

Increased Inhibitory Cell-Cell Communication Between High OSBPL3 Expression Tumor and Immune Cells. (A) Dimensionality reduction and clustering of single-cell pancreatic cancer data from the GSE155698 dataset. (B) Volcano plot showing differentially expressed genes between OSBPL3 high-expression and low-expression ductal cell subpopulations. Genes upregulated in the low-expression group are on the left, while those upregulated in the high-expression group are on the right. (C) GO pathway enrichment analysis of differentially expressed genes in OSBPL3 high- and low-expression ductal cell subpopulations. Blue bars represent pathways enriched in the high-expression group, and red bars represent pathways enriched in the low-expression group. The x-axis indicates -Log10(p-value). (D) GSEA analysis of WNT-related pathways following OSBPL3 knockdown in the CFPAC-1 cell line. (E) Western blot analysis of WNT pathway gene expression after OSBPL3 knockdown or overexpression. (F) Expression changes of various WNT-related genes following OSBPL3 knockdown in the CFPAC-1 cell line. The x-axis represents gene names, and the y-axis shows the Log2FC values for differential expression between the knockdown and control groups. (G) Expression of key WNT pathway genes in OSBPL3 high- and low-expressing cell populations from the single-cell pancreatic cancer dataset GSE155698. (H) Violin plot showing the expression of WNT pathway-related immunosuppressive cytokines in macrophages, epithelial cells, and T cells with high or low OSBPL3 expression. (I) Cell-cell communication intensity across different cell subpopulations. Different colors represent signals emitted by various cell subpopulations. (J) Bubble plot depicting the expression of ligands generated by OSBPL3 high-expressing ductal cells across different cell subpopulations. (K) A schematic illustrating the role of high OSBPL3 expression in contributing to the immunosuppressive microenvironment in pancreatic cancer