Fig. 5

METTL7B inhibited tumor growth and ferroptosis in xenograft tumor model induced by Erastin. (A) Tumor growth curves of xenograft tumors derived from SW780 cells with METTL7B knockdown. (B) Representative images of xenograft tumors (left panel) and tumor weights (right panel) of xenograft from SW780 cells in nude mice. (C) Immunohistochemical analysis of METTL7B, Ki67, and ACSL3 in SW780 xenograft tumors. Scale bar = 50 μm. (D) The protein expression levels of ACSL3 in tumor. E-F) Ferrous iron (E) and MDA (F) content in SW780 xenograft tumors was detected using corresponding commercial kits. METTL7B: methyltransferase-like 7B. ACSL3, acyl-coA synthetase long chain family member 3. MDA: malondialdehyde. shnc: negative control short hairpin RNA. shMETTL7B: short hairpin RNA against METTL7B. ^*P < 0.05, ^**P < 0.01 versus shnc. ^{^}P < 0.05 versus Erastin + shnc