Fig. 6

Involvement of PLAGL2 in METTL7B transcriptional regulation. A-B) Expression (A) of PLAGL2 and the correlation analysis (B) between PLAGL2 and METTL7B in bladder cancer from UALCAN database. Pearson Correlation Coefficient R = 0.34, P < 0.05. C) Efficiencies of PLAGL2 knockdown or overexpression were validated by RT-qPCR in SW780 cells. ^**P < 0.01 versus nc. ^{^}P < 0.05 versus vector. D) RT-qPCR and Western blot measurement of METTL7B expression in SW780 cells with PLAGL2 knockdown or overexpression. ^**P < 0.01 versus nc. ^{^}P < 0.05 versus vector. E) METTL7B promoter activity upon PLAGL2 overexpression, as measure by dual luciferase method. ^**P < 0.01 versus vector. F) ChIP assay of PLAGL2 binding to METTL7B promoter. PLAGL2: PLAG1 Like Zinc Finger 2. METTL7B: methyltransferase-like 7B. UACLAN: University of Alabama at Birmingham Cancer Data Analysis Portal. ChIP: chromatin immunoprecipitation. nc: negative control. siPLAGL2#1/2: small interfering targeting PLAGL2. oe-PLAGL2: PLAGL2 overexpression plasmid. bd: potential binding domain. LUC: luciferase