Fig. 1
BRCA1 is identified as a highly expressed hub gene in the AcASCs. A Initiation of antler regeneration is a process from PoPP to AcPP. B Module analysis of all up-regulated DEGs between the AcPP and PoPP. C Venn diagram showing 39 overlapping (hub) genes applying two different calculation methods (CytoNCA and Cytohubba) for the up-regulated DEGs. D Correlation Heatmap of the 39 hub genes (P value < 0.05). E Enrichment results of GO biological process and KEGG pathways constructed using 39 hub genes via ClueGO and CluePedia plugins. F Immunofluorescent staining of BRCA1 (white arrows) in the AcPP and PoPP tissues. G Percentage of BRCA1 positive cells. Data are shown as the mean ± SEM, n = 6, **p < 0.01; ****p < 0.0001. H Relative expression levels of BRCA1 mRNA in the AcAnSCs, the PoAnSCs and FPCs. Data are shown as the mean ± SEM, n = 3, **p < 0.01; ****p < 0.0001