Fig. 3

BRCA1 deletion promoted apoptosis and senescence of the AcAnSCs. A Cell apoptosis analysis using flow cytometry. B Cell senescence analysis via β-galactosidase staining. C Percentage of early-apoptotic and late-apoptotic cells in the different groups; note that numbers of early-apoptotic and late-apoptotic cells in the BRCA1-/- group were significantly higher than those in the Control and Sham groups; data are shown as the mean ± SEM, n = 3, ****p < 0.0001. D Percentage of senescent cells in the different groups; the results showed that the percentage of aging cells following BRCA1 knockout was significantly increased compared to the Control and Sham groups. Data is shown as the mean ± SEM, n = 6, ***p < 0.001; ****p < 0.0001