Fig. 4

BRCA1 deletion in the AcAnSCs increased DNA damage and UV-induced apoptosis. A Relative expression levels of BRCA1 mRNA in the different groups after UV treatment; data are shown as the mean ± SEM, n = 3, ***p < 0.001; ****p < 0.0001. B, C Immunofluorescent staining of γ-H2AX. D The number of γ-H2AX foci per cell in the different groups; note that the number ofγ-H2AX foci per cell was higher in the BRCA1-/- group than in the Control and Sham groups; data are shown as the mean ± SEM, n = 5, **p < 0.01. E Percentage of γ-H2AX positive cells (foci > 50); note that the percentage of γ-H2AX positive cells was significantly higher in the BRCA1-/- group than in the Control and Sham groups; data are shown as the mean ± SEM, n = 5, *p < 0.05,***p < 0.001,****p < 0.0001. F Western blot analysis of γ-H2AX protein expression. G Relative expression levels of γ-H2AX protein; data are shown as the mean ± SEM, n = 3, *p < 0.05. H Cell apoptosis analysis using flow cytometry after UV-treatment. I Percentage of apoptotic cells in the different groups; data are shown as the mean ± SEM, n = 3, *p < 0.05, **p < 0.01