Fig. 6

BRCA1 deletion induced excessive endoplasmic reticulum (ER) stress response. A Relative expression levels of BRCA1 mRNA in the different groups after TM treatment; data are shown as the mean ± SEM, n = 3, **p < 0.01, ****p < 0.0001. B Western blot analysis of GRP78 protein expression. C and D Relative expression levels of GRP78 protein (C) and mRNA (D); note that both protein and mRNA levels in the BRCA1-/- group were higher than in the other groups; data are shown as the mean ± SEM, n = 3, *p < 0.05, ***p < 0.001. E Cell apoptosis analysis via flow cytometry after TM-treatment. F Percentage of apoptotic cells in the different groups; note that apoptotic cells in the BRCA1-/- group after TM-treatment were significantly higher than those in the other two groups; data are shown as the mean ± SEM, n = 3, *p < 0.05, ***p < 0.001