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Fig. 6 | Biology Direct

Fig. 6

From: Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation

Fig. 6

Knockdown of DOCK2 suppressed LPS-induced M1 polarisation of mouse macrophages. (A) Schematic illustration of experimental protocols. (B) Protein levels of DOCK2 6 h after 100 ng/mL LPS stimulation. (C) Relative mRNA level of DOCK2 in RAW264.7 cells 48 h after adenovirus transduction. (D) 48 h after adenovirus transduction, cells were stimulated with 100 ng/mL LPS. Representative microphotographs of IF staining of iNOS 6 h later. Magnification: 400 fold. Scale bar: 50 μm. (E) Relative mRNA levels of M1 macrophage cytokines (IL-6, TNF-α, and iNOS). (F) Representative microphotographs of IF staining of GTP-RAC1 in cells. Magnification: 400 fold. Scale bar: 50 μm. The quantification of mean fluorescence intensity (MFI) is shown in the right panel. (G) The pull-down assay of GTP-RAC1 by PAK-PBD protein beads. (H) Protein levels of DOCK2 in cells

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