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Fig. 7 | Biology Direct

Fig. 7

From: Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation

Fig. 7

Knockdown of DOCK2 inhibits TGF-β1-induced activation of HSC cells. (A) Schematic illustration of experimental protocols. (B) Protein levels of DOCK2 24 h after 10 ng/mL TGF-β1 stimulation. (C) Relative mRNA levels of DOCK2 in HSCs 48 h after adenovirus transduction. (D) 24 h after adenovirus transduction, cells were stimulated with 10 ng/mL TGF-β1. Representative microphotographs of IF staining of α-SMA 24 h later. Magnification: 400 fold. Scale bar: 50 μm. (E) Protein levels ofα-SMA in cells. (F) Representative microphotographs of IF staining of GTP-RAC1 in cells. Magnification: 400 fold. Scale bar: 50 μm. (G) The quantification of MFI. (H) The pull-down assay of GTP-RAC1 by PAK-PBD protein beads. (I) Protein levels of DOCK2 in cells. (J) Venn analysis showing the common differentially expressed genes (DEGs;|Log2FC|>1 and p < 0.05) between DOCK2-silenced M1 macrophages and myofibroblasts. (K) GO biological processes and KEGG pathways enriched for these common DEGs

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