Fig. 3
From: Oncogenic RIT1 mutations confer ferroptosis vulnerability in lung adenocarcinoma
Depletion of RIT1 inhibits proliferation of lung adenocarcinoma cells and RIT1 A77, F82 and M90 mutations promote cell growth. (A) Correlation analysis of RIT1 alterations with those of its RAS paralogs in lung adenocarcinoma with cBioPortal platform (https://www.cbioportal.org/). (B and C) The efficiency of RIT1 knockdown in A549 and H1975 cells was validated by immunoblotting. Tubulin or GAPDH was used as protein loading control. Below images and column charts show representative colony formation assays and quantification of colony number and size. (D) Colony formation assays performed using HBE135-E6E7 stable cell lines with WT or mutated RIT1 overexpression as indicated. Quantification was conducted using ImageJ software. Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. n.s., not significant