Fig. 3

Analysis of the YSD plasmids in natural yeast strains. (a) Total proteins from 8 natural yeast strains (IB1-IB8), carrying the pYSD-yCup1 plasmid, were analyzed by Western blot as indicated in Fig. 2. (b) Western blot assay performed for the IB1-IB3 natural strains transformed with pYSD-6xHis. The GAPDH protein was used as loading control. Images are representative of three independent experiments. (c) Yeast IB1-IB3 strains carrying either pYSD-yCup1, or pYSD-6xHis plasmids, were analyzed by immunofluorescence microscopy to visualize the localization of fusion proteins. For each strain, micrographs are reported of the differential interference contrast (DIC), the immunofluorescence signal (AF555), the nuclear DNA staining (DAPI), and the merged image. Images are representative of three biological replicates for each yeast strain. Scale bar = 5 μm