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Fig. 2 | Biology Direct

Fig. 2

From: Nucleolin in the cell membrane promotes Ang II-mediated VSMC phenotypic switching by regulating the AT1R internalization function

Fig. 2Fig. 2

Ang II promotes VSMC phenotype switching through NCL. A. RT-qPCR and WB analysis of NCL expression in VSMCs transfected with sh-NC or sh-NCL. *p < 0.05 vs. sh-NC. B-C. RT-qPCR and WB analysis of NCL expression in VSMCs. D. CCK-8 assay for evaluating cell viability of VSMCs. E. EDU assay for detecting cell proliferation in VSMCs. Scale bar = 50 µM. F. Scratch assay for detecting cell migration in VSMCs. Scale bar = 100 µM. G. WB analysis of α-SMA, SM22α, and OPN expression. H. IF assay for detecting α-SMA and OPN expression in VSMCs. Scale bar = 25 µM. VSMCs were transfected with sh-NC or sh-NCL and then treated with Ang II. *p < 0.05 vs. Control, #p < 0.05 vs. Ang II + sh-NC. n = 3

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