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Fig. 5 | Biology Direct

Fig. 5

From: Nucleolin in the cell membrane promotes Ang II-mediated VSMC phenotypic switching by regulating the AT1R internalization function

Fig. 5

NCL delays Ang II-induced AT1R internalization and recruits Rab4 and Rab11 to promote recycling by inhibiting AT1R phosphorylation. A. RT-qPCR and WB analysis of NCL expression in VSMCs transfected with oe-NC or oe-NCL. *p < 0.05 vs. oe-NC. B. WB analysis of AT1R expression in the cell membrane and cytoplasm of VSMCs. C. WB analysis of β-arrestin2, Clathrin, AP-2, and Caveolae-1 expression in VSMCs. D. Detection of AT1R phosphorylation in VSMCs. Pulldown Westerns are indicated by pulldown (PD). E. Co-IP assay for validating the binding of AT1R to Rab4 and Rab11, respectively. F. WB analysis of Rab4 and Rab11expression in VSMCs. VSMCs were transfected with oe-NC or oe-NCL and then treated with Ang II. *p < 0.05 vs. Control, #p < 0.05 vs. Ang II + oe-NC. n = 3

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