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Fig. 6 | Biology Direct

Fig. 6

From: Nucleolin in the cell membrane promotes Ang II-mediated VSMC phenotypic switching by regulating the AT1R internalization function

Fig. 6Fig. 6

NCL induces VSMC phenotypic switching by reducing Ang II-induced AT1R internalization leading to sustained AT1R activation. VSMCs were transfected with oe-NC or oe-NCL and then treated with Ang II and 50 μm rasarfin. A. WB analysis of β-arrestin2, Clathrin, AP-2, and Caveolae-1 expression in VSMCs. B. Detection of AT1R phosphorylation in VSMCs. C. WB analysis of Rab4 and Rab11expression in VSMCs. D. EDU assay for detecting cell proliferation. Scale bar = 50 µM. E. Scratch assay for detecting cell migration. Scale bar = 100 µM. F. WB analysis of α-SMA, SM22α, and OPN expression. G. IF assay for detecting α-SMA and OPN expression. Scale bar = 25 µM. VSMCs were transfected with sh-NC or sh-NCL and then treated with Ang II and rasarfin. *p < 0.05 vs. Ang II + sh-NC, #p < 0.05 vs. Ang II + sh-NCL + DMSO. n = 3

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