Fig. 2

HOXB4 inhibits proliferation and promotes apoptosis of hepatocellular carcinoma cell in vitro. (a-b) The proliferation of HCC cells with upregulated or downregulated HOXB4 expression; (c-d) EdU assay detected the proliferation of HCC cells with upregulated or downregulated HOXB4 expression. Scale bar, 50 μm; (e-f) Colony formation ability of HCC cells with upregulated or downregulated HOXB4 expression; (g) Representative immunoblots of PCNA in Li-7 cells with upregulated or downregulated HOXB4 expression; (h) Cell apoptosis of Li-7 cells with upregulated HOXB4 expression according to flow cytometry analysis; (i) Representative immunoblots of Bax, Bcl-2, cleaved caspase 3, and cleaved caspase 9 protein levels in Li-7 cells with upregulated HOXB4 expression. Data are expressed as mean ± SD, N = 3. ^**p < 0.01, ^***p < 0.001 vs. EV^Tet+dox; ^#p < 0.05, ^##p < 0.01 vs. shNC^Tet+dox