Fig. 3

HOXB4 suppresses migration and invasion via inhibiting EMT of hepatocellular carcinoma cell. (a-d) Representative images of wound healing experiments and quantitative data of migration of HCC cells with upregulated or downregulated HOXB4 expression. Scale bar, 200 μm; (e-f) Invasion ability of HCC cells with upregulated or downregulated HOXB4 expression was determined by Transwell assays. Scale bar, 100 μm; (g) Immunoblots analysis of active MMP-2, active MMP-9, E-cadherin, N-cadherin, and Vimentin in Li-7 cells with upregulated or downregulated HOXB4 expression; (h) Representative immunofluorescence images of E-cadherin expression in Li-7 cells with upregulated or downregulated HOXB4 expression. Scale bar, 50 μm. Data are expressed as mean ± SD, N = 3. ^**p < 0.01, ^***p < 0.001 vs. EV^Tet+dox; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. shNC^Tet+dox