Fig. 6

METTL7B is a target gene of HOXB4 and functions in hepatocellular carcinoma. (a) Principal component analysis (PCA) of HOXB4-overexpressed Li-7 cells and its control cells; (b) Heatmap showing DEGs in HOXB4-overexpressed Li-7 cells.|Log2FC| > 1 and adj. p < 0.005 were cutoff values; (c) The gene expression levels of m6A regulators in HCC using TNMplot; (d) Heatmap showing expression of methyltransferase-like proteins in HOXB4-overexpressed Li-7 cells; (e) Representative immunoblots of METTL7B protein in HCC cells with upregulated or downregulated HOXB4 expression; (f) Dual-luciferase assay determine the binding of HOXB4 to METTL7B promoter regions in HCC; (g) Schematic of the METTL7B gene showing sequence locations of ChIP primer pairs; (h) ChIP results of HOXB4 binding to the promoter region of METTL7B; (i) The binding of HOXB4 and METTL7B was performed using Pull-down assay; (j) Representative immunoblots of METTL7B in Li-7 cells with METTL7B and HOXB4 knockdown; (k) Cell viability of Li-7 cells with METTL7B and HOXB4 knockdown; (l) The invasion ability of Li-7 cells with METTL7B and HOXB4 knockdown. Scale bar, 100 μm. (m) Representative immunoblots of PD-L1 in Li-7 cells with METTL7B and HOXB4 knockdown. Data are expressed as mean ± SD, N = 3. ^**p < 0.01, ^***p < 0.001