Fig. 2

Single-cell atlas of cardiac cells and infiltrating immune cells in VMC heart, and functional analysis of macrophages. (A) Schematic diagram showing scRNA-seq pipeline of cardiac tissues. (B) UMAP plot of cardiac cells and infiltrating immune cells colored by cell type. (C) Bubble plot of marker genes of each cell type. The dot color represents the gene expression level, and the dot size represents the percentage of cells expressing the respective gene. (D) Histogram of all cell population proportions (left) and doughnut chart of immune cell population proportions (right). (E) Representative immunofluorescent staining of macrophages (CD68, green) and nuclei (DAPI, blue) in cardiac tissues from control and VMC mice. Scale bars: 50 μm. (F) Top 15 upregulated GO analysis terms of differentially expressed genes (DEGs) between control and VMC mice in macrophages. DEGs used in the analysis were selected with P < 0.01 and|log2FC| ≥ 0.26. (G) KEGG analysis of DEGs between control and VMC mice in macrophages. (H) Interaction strength of ligand–receptor pairs between macrophages and other cell populations in the myocarditis group