Fig. 5

Macrophage-derived SPP1 upregulates the expression of Ccl2 and Ccl7 in cardiac fibroblasts in vitro. (A) The expression levels of SPP1 in the supernatant of BMDMs with or without CVB3 stimulation. (B) Western blotting analysis of SPP1 in BMDMs with or without CVB3 stimulation. (C) Schematic diagram showing the process of co-culture of BMDMs and fibroblasts with CVB3 stimulation and SPP1 inhibition. (D) The expression levels of Spp1 in the supernatant of BMDMs from control, CVB3 and SEI + CVB3 groups. (E) Western blotting analysis of SPP1 in BMDMs from control, CVB3 and SEI + CVB3 groups. (F) Relative Ccl2 and Ccl7 mRNA levels of cardiac fibroblasts from control, CVB3 and SEI + CVB3 groups. Unpaired t-test (two groups) and ANOVA (three groups) was used for analysis. Data are presented as mean ± SD (n = 3 biologically independent samples). ***P < 0.001, **P < 0.01, *P < 0.05, ns P > 0.05