Fig. 6

Macrophage depletion at an early stage alleviates myocardial inflammation in VMC mice: (A) Timeline of macrophage depletion treatment. (B) Ratio of macrophages in spleen from control, CVB3 and Clodronate + CVB3 mice. (C) Weight changes of the three groups in the following 7 days after treatment. (D) Representative TUNEL staining of heart sections from control, CVB3 and Clodronate + CVB3 mice. (E) Representative parasternal long axis 2-dimensional and M-mode echocardiography images from control, CVB3 and Clodronate + CVB3 mice. (F) Cardiac immune infiltration status in different groups. Scale bars: 50 μm. (G) EF and FS of control, CVB3 and Clodronate + CVB3 mice. (H) Myocarditis scores for cardiac immune infiltration from control, CVB3 and Clodronate + CVB3 mice. (I) The levels of inflammatory cytokines (IL-1β, IL-6, TNF-α), cTnT and BNP in plasma from control, CVB3 and Clodronate + CVB3 mice. (J) Relative mRNA levels of inflammatory factors (IL-1β, IL-6, TNF-α, IFN-γ), Ccl2 and Ccl7 of cardiac tissues from control, CVB3 and Clodronate + CVB3 mice. ANOVA was used for analysis. Data are presented as mean ± SD (n = 5 biologically independent samples). ***P < 0.001, **P < 0.01, *P < 0.05, ns P > 0.05