Fig. 7

Macrophage depletion reduces SPP1 levels in VMC mice: (A) The Western blot results of SPP1 protein expression in cardiac tissues from control, CVB3 and Clodronate + CVB3 mice. (B) The quantitative analysis for Western blot results of SPP1 protein expression in the cardiac tissues. (C) Relative Spp1 mRNA levels of cardiac tissues from control, CVB3 and Clodronate + CVB3 mice. (D) The expression levels of SPP1 in plasma from control, CVB3 and Clodronate + CVB3 mice. (E) Representative immunofluorescent staining of macrophages (CD68, green), nuclei (DAPI, blue) and SPP1 (red) in cardiac tissues from control, CVB3 and Clodronate + CVB3 mice. Scale bars: 125 μm. ANOVA was used for analysis. Data are presented as mean ± SD (n = 5 biologically independent samples). ***P < 0.001, **P < 0.01, *P < 0.05, ns P > 0.05