Fig. 2
Colon cancer cell-derived exosomes polarize M1 macrophage into M2 phenotype in vitro. (A-C) Mouse M1 macrophages were treated with 20 µg of CT-26 cell-derived exosomes (CT-26/exo) or MCEC cell-derived exosomes (MCEC/exo) for 48 h. Cells were subjected to qPCR (A) or western blot (B-C) analyses (n = 3 independent experiments). (D-E) Mouse M1 macrophages were treated with 20 µg of CT-26/exo, MCEC/exo, or PBS or were treated with 40 µg of CT-26/exo (CT-26/exo High) for 48 h. Cells were subjected to FACS analyses (n = 3 independent experiments). (F) Mouse M1 macrophages were treated with 20 µg of CT-26/exo, MCEC/exo for 48 h followed by examination of as indicated cytokines levels in the medium (n = 3 independent experiments). (G-H) Mouse M1 macrophages (1 × 106) were treated with 20 µg of CT-26/exo, MCEC/exo for 48 h, followed by co-culture with CD8 + T cells (1 × 106) derived from mouse spleen for 48 h. Cells were subjected to FACS analyses (n = 3 independent experiments). Data were presented as mean ± SD (A, C, E, F, H). Statistical analyses were performed with one-way ANOVA with Tukey’s test (A, C, E, F, H)