Fig. 4

A, B Huh7 and HepG2 cells with Ref-1 knockdown were treated with L-741626. Compared with the nonknockdown control group the sensitivity of Huh7 and HepG2 cells to L-741626 was reduced following Ref-1 knockdown. C western blot experiments were used to detect the expression levels of ERK1/2 and p-ERK1/2 in Huh7 and HepG2 cells with Ref-1 knockdown. D Analysis of the correlation between Ref-1 and CRAF protein expression in HCC was conducted using the GEPIA2 database. E Coimmunoprecipitation (co-IP) experiments demonstrated that Ref-1 and CRAF can directly bind to one another. F L-741626 targets Ref-1 and modulates the interaction between Ref-1 and CRAF, thereby inhibiting the MAPK/ERK signalling pathway. The data are presented as means ± SDs; * P < 0.05; ** P < 0.01; ***P < 0.001