Fig. 6

Biological activity of selected tulip PEBPs. (A) Y2H assay showing the protein–protein interaction capacity of tulip PEBPs with tulip and Arabidopsis FD and TB1/BRC1-like proteins. EV is a negative control based on the introduction of an Empty AD or BD vector. Full Y2H data can be found in Supplementary Fig. 7. (B) Percentage of active rosette axillary buds in Arabidopsis lines constitutively expressing TgFT1, TgFT2, TgFT3, or TgFT4. Plants were scored at the end of their lifecycle, at the first visible signs of silique browning. (C) Flowering time of Arabidopsis lines constitutively expressing TgFT1, TgFT2, TgFT3, or TgFT4, measured in the number of rosette leaves at visible bolting. (D) Morphology of Arabidopsis lines constitutively expressing TgFT5, at the stage of anthesis. The white bar indicates 1 cm. Asterisks indicate p < 0.05 in pairwise T-test assuming equal variance