Fig. 5

LASSO regression analysis identifies key genes in the progression of DFU, including SH3BGRL3. A Comparison of GSVA scores for 50 candidate hub genes between the DFU group and the control group. B Changes in the coefficients of selected features using the LASSO algorithm. C Feature selection using the LASSO algorithm. D UMAP plots of SH3BGRL3 and TMSB10 at the single-cell sequencing level. E Expression of SH3BGRL3 and TMSB10 in different groups from single-cell sequencing. F, G Western blotting was used to detect the expression levels of SH3BGRL3, VEGF-A, HIF-1α, ERK, and AKT in HUVECs under high-glucose (HG) conditions and after overexpression of SH3BGRL3. Data are presented as mean values ± standard deviation, n = 3, ** p < 0.01, # p < 0.05, ## p < 0.01, ### p < 0.001