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Fig. 5 | Biology Direct

Fig. 5

From: c-Jun promotes neuroblastoma cell differentiation by inhibiting APC formation via CDC16 and reduces neuroblastoma malignancy

Fig. 5

c-Jun interacted with CDC16 and inhibited its interaction with CDC20 and CDC27. (A). Proteins interacting with c-Jun were detected by co-immunoprecipitation (Co-IP) and silver staining in c-Jun OE cells. (B). Different protein bands in IgG group (NC) and c-Jun antibody group (IP) were analyzed by mass spectrometry, and the number of different proteins in the two groups were shown by Venn diagram. (C-D). Co-IP assay with c-Jun antibody (C) and CDC16 antibody (D) to confirm the interaction of CDC16 with c-Jun. IgG were used as negative control. (E). Co-IP assay with CDC16 antibody to detect the interaction between CDC16 and c-Jun or the interaction between CDC16 and CDC20/CDC27 in negative control (NC, cells transfected with Lentiviral vector) and c-Jun OE SH-SY5Y cells. (F). Relative protein level precipitated by CDC16 antibody. The ratio of IP group to total protein was statistically analyzed by t test (*p < 0.05, **p < 0.01, ***p < 0.001) and the results were presented as mean ± SEM (n = 3)

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