Fig. 6

CDC16 overexpression abolished cell differentiation mediated by c-Jun overexpression. (A). Immunoblots showing the expression of differentiation markers in control cells (NC), c-Jun overexpressed cells (c-Jun OE) and cells co-overexpressed c-Jun and CDC16 (c-Jun OE + CDC16 OE) after RA-induced differentiation. (B-D). Relative protein level of TUBB3 and GAP43 (ratio to GAPDH) analyzed by ordinary one-way ANOVA combined with Sidak’s multiple comparisons test. (E). Neural differentiation of NC, c-Jun OE and c-Jun OE + CDC16 OE SH-SY5Y cells. Cells were stained with TUBB3 and DAPI and images were acquired using a fluorescence microscope (Nikon). Number of cells with neurite outgrowth (> 20 μm) was counted using Image J software with cell counter plugin. Data were analyzed by ordinary one-way ANOVA combined with Sidak’s multiple comparisons test (mean ± SEM, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). Scale bar: 50 μm