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Table 3 Average Tm1 values of hINMT in response to SAM and SAH

From: Functional and structural characterization of the human indolethylamine N-methyltransferase through fluorometric, thermal and computational docking analyses

 

SAM

(ΔTm1 =  + 7.2 °C)

SAH

(ΔTm1 =  + 8.5 °C)

0 µM

43.3 ± 0.2 °C

43.3 ± 0.2 °C

4 µM

44.3 ± 0.2 °C

44.3 ± 0.2 °C

8 µM

45.2 ± 0.2 °C

45.3 ± 0.2 °C

16 µM

46 ± 0 °C

46 ± 0 °C

32 µM

46.5 ± 0 °C

46.7 ± 0.2 °C

64 µM

47.3 ± 0.2 °C

47.3 ± 0.2 °C

128 µM

48 ± 0 °C

48.2 ± 0.2 °C

256 µM

49 ± 0 °C

49 ± 0 °C

512 µM

49.8 ± 0.2 °C

49.8 ± 0.2 °C

1024 µM

50.7 ± 0.2 °C

50.5 ± 0 °C

2048 µM

50.5 ± 0 °C

51.8 ± 0.2 °C

  1. The Tm is determined at the first peak of each fluorescence derivative curve (Tm1) illustrated in Figure S5. The results related to tryptamine and quinoline are omitted due to no significant effects