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Fig. 1 | Biology Direct

Fig. 1

From: The CXCR3/PLC/IP3-IP3R axis is responsible for the ignition of UPR in intestinal epithelial cells exposed to gliadin peptide, during the onset of celiac disease

Fig. 1

The PLC/IP3/IP3R axis stimulated by PT induces ER stress in IEC. CaCo-2 cells were loaded with Fura-2/AM (2.5 μM) and exposed to 1 mg/ml PT alone (PT) or in combination with AMG 487 (1 μM; PT + AMG) or U73122 (10 μM; PT + U) and Fura-2 fluorescence was recorded and expressed as Fura-2 ratio (A). Data are expressed as mean of peak ± SD. Cells were unexposed or exposed to PT (1 mg/mL; 9 h) alone or in combination with U73122 (U, 10 μM; 1 h pretreatment), and the expression of ATF4, ATF6, XBP1s (B), and TG2 (C) was evaluated by qPCR. S.i. from GF mice were cultivated in the GEVS and untreated or treated with PT (5 mg/mL) alone or in combination with U73122 (U, 20 μM; 2 h pretreatment) and the expression of ATF4, ATF6, XBP1s (D), and TG2 (E) was evaluated by qPCR

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