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Fig. 2 | Biology Direct

Fig. 2

From: The CXCR3/PLC/IP3-IP3R axis is responsible for the ignition of UPR in intestinal epithelial cells exposed to gliadin peptide, during the onset of celiac disease

Fig. 2

CXCR3 is stimulated by PT and induces ER stress in IEC. The expression of CXCR3 was evaluated in both CaCo-2 cells (A) or s.i. (B) unexposed or exposed to PT (1 mg/mL, 9 h, in CaCo-2; 5 mg/mL, 16 h, in GEVS) by western blotting analysis. Cells were unexposed or exposed to PT (1 mg/mL; 9 h) alone or in combination with AMG 487 (AMG, 1 μM; 8 h pretreatment), and the expression of indicated ER stress markers was evaluated by qPCR (C). S.i. from GF mice were cultivated in the GEVS and untreated or treated with PT (5 mg/mL) alone or in combination with AMG 487 (AMG, 1 μM; 16 h pretreatment) and the expression of ATF4, ATF6, XBP1s was evaluated by qPCR (D). The production of pro-inflammatory cytokines was evaluated by ELISA (E), while tissue permeability was evaluated by using the FITC-Dextran protocol (F), in the same experimental conditions

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