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Fig. 3 | Biology Direct

Fig. 3

From: The CXCR3/PLC/IP3-IP3R axis is responsible for the ignition of UPR in intestinal epithelial cells exposed to gliadin peptide, during the onset of celiac disease

Fig. 3

CXCR3 is strictly required to induce a PT-dependent ER Stress. Basal expression of CXCR3 was evaluated in both CaCo-2 and HEK 293 cells by qPCR (A, left panel) or western blotting (A, right panel) analysis. HEK cells were unexposed or exposed to TG (10 μg/ml, 3 h) or PT (1 mg/ml, 3–6 h), and the expression of CXCR3 (B), or indicated ER stress markers (C) was evaluated by qPCR. CXCR3 was stably overexpressed in HEK cells and both protein (D) and mRNA (E) levels were evaluated by western blotting or qPCR, respectively. Parental (HEK) and CXCR3 overexpressing (HEKCXCR3) HEK cells were unexposed or exposed to PT (1 mg/ml, 3 h), and the expression of indicated ER stress markers was than evaluated by qPCR (F). Endogenous CXCR3 was evaluated in both parental and HEKCXCR3 exposed to PT (1 mg/ml, 3 h), by qPCR (G)

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