Fig. 1

DNM1L promoted GBC cell proliferation and inhibited cell apoptosis. a The gain- and loss-function assay were designed using the Dox-inducible system in GBC-SD and NOZ cells. b After treating with or without Dox for 48 h, the cell viability of GBC-SD and NOZ cells was detected by CCK-8 assay and represented as OD values at 450 nm. c EDU staining was used to detect cell proliferation, and d the EDU-positive cells (red fluorescence) were quantified. e The distribution of cells across the various phases of the cell cycle was assessed using flow cytometry. f Western blot analysis for cell cycle markers, including Cyclin E and p21. g Quantitative analysis of the cell cycle markers. h Statistical data showed the cell percentage of early apoptosis (AnnexinV-FITC⁺PI⁻ cells) and late apoptosis (AnnexinV-FITC⁺PI⁺ cells). i Western blot analysis for the cleaved PARP and caspase 3. n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001