Fig. 3

DNM1L disrupted mitochondrial homeostasis and induced mitochondrial dysfunction in GBC. a Four important indicators of mitochondrial homeostasis were associated with the cancer progression. b The content of ATP in cells was detected and corrected according to the protein concentration. c Flow cytometry was performed to detect the ROS level of GBC cells. d The morphology of mitochondria was observed by transmission electron microscopy. e The mitochondrial network stained with Mitotracker red to identify mitochondrial fragmentation was visualized under microscopy, and f the fluorescent intensity was shown. g Cytosolic mtDNA copy number was quantitated via qRT-PCR assay in GBC cells. n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001