Fig. 5

A USP3 interacted with DNM1L and enhanced its stability. a qRT-PCR and b western blot analysis for the DNM1L expression after USP3 overexpression or knockdown. c Co-IP assay detected the direct interaction between USP3 and DNM1L in GBC cells. d NOZ cells transfected with the indicated plasmids were treated with cycloheximide (CHX) for 0, 1, 3, 6, and 9 h, and they were collected for western blot analysis. e USP3 mediated deubiquitination and K48-linked deubiquitination of DNM1L were detected. Co-IP assays were performed with the indicated antibodies. (f) To identify the region of each protein responsible for the interaction, HA-tagged DNM1L WT or truncation mutants were co-expressed with Flag-USP3 in HEK293 cells. n = 3 per group. **p < 0.01, ***p < 0.001