Fig. 5

Single-cell landscape of macrophage subpopulations in LTS. (a) Ridge plots displayed the expression signatures of 10 common myeloid lineage-specific markers for each cell type. The curves represented the KDE, with the height indicating the density in the region. (b) UMAP plots showed 16 distinct myeloid cell clusters identified during LTS progression, with each cluster annotated by a different color. (c) Volcano plot highlighted the top 5 DEGs in each myeloid cell cluster. The Y-axis represented the log2-fold change, indicating the magnitude of gene expression change, while the X-axis reflected the percentage of cells expressing each DEG. (d) UMAP plots demonstrated 9 distinct myeloid cell subpopulations found during LTS procession. Each subpopulation was annotated by a different color. (e) Bar chart shown the fraction of myeloid cell subpopulations at each time point. Blocks represented distinct time points: LTS_1(1 day after LTS induction), LTS_3 (3 days), LTS_5 (5 days), and LTS_7 (7 days). The block height was proportional to the number of cells at each time point. (f) Biological functional scores showed the M1/M2 polarization scores for each cell cluster. The middle horizontal line represented the median, with the upper and lower edges of the box indicating the third and first quartiles, respectively. (g) Dot plot illustrated the gene expression signatures of myeloid cell subpopulation. Dot color intensity represented the average expression level, ranging from low to high and dot size was proportional to the fraction of cells expressing the gene