Fig. 3
From: GAMT facilitates tumor progression via inhibiting p53 in clear cell renal cell carcinoma
GAMT regulates ccRCC independent of its enzyme activity. A, B. Creatine levels in RCC cells with GAMT knockdown or overexpression were validated using ELISA (A) and LC–MS (B). C. Effect of cyclocreatine (CCr) on creatine levels in GAMT high-expression ccRCC cells and GAMT overexpression ccRCC cells validated using ELISA. D. CCK-8 assay was performed on ccRCC cells stably expressing mutated or wild-type GAMT. E. Representative images of migrated ccRCC cells overexpressing GAMT and GAMT overexpression ccRCC cells stably expressing mutant GAMT via wound healing. F. Representative images of migrated or invaded ccRCC cells overexpressing GAMT and GAMT overexpression ccRCC cells stably expressing mutant GAMT via Transwell assays. ** p < 0.01, *** p < 0.001