Fig. 5

Impact of HOMER3 knockdown on glioma cell proliferation and cell proliferation regulators. (A) Elevated HOMER3 expression levels in five glioma cell lines compared to normal cells, confirmed by RT-PCR and western blot analysis. (B) Efficacy of HOMER3 knockdown using shRNA plasmids. (C-D) Cell proliferation assessment via CCK-8 assays demonstrating significant reductions in proliferation rates post-HOMER3 knockdown in U251 and U87 glioma cells. (E) In vivo effects of HOMER3 knockdown using a subcutaneous xenograft mouse model, illustrating reduced tumor size and weight compared to negative control groups. Each group contained n = 6 mice, and the experiment was repeated three times independently. (F) IHC staining of Ki-67 and PCNA confirming diminished proliferation in HOMER3 knockdown tumors. (G-I) Correlation analyses revealing relationships between HOMER3 expression and key cell proliferation regulators, indicating HOMER3’s influence on cell cycle progression. (J) Western blot analysis showing shifts in expression patterns of CDK4, CDK6, cyclin D1, and p27 following HOMER3 knockdown. All experiments were performed in three independent biological replicates (n = 3) to ensure the reproducibility and reliability of the results