Fig. 1

Atherosclerosis-induced ApoE KO mice were fed a high-fat diet. A. Representative images of Aortic Oil Red O staining from young and aged atherosclerotic ApoE null mice fed a regular or a high-fat diet for 8 weeks (N = 3 mice/group. Mean ± ScD. *p < 0.05 young vs. aged aortas). B. Representative Eosin/Hematoxilin staining of aortic sections of the same mice as in (A) (N = 3 Mean ± SD. *p < 0.01 Young vs. Aged). C. Representative β-galactosidase staining of P0 HAEC incubated with EVs from aged and young ApoE null mice and isolated at indicated times (N = 3 Mean ± SD. *p < 0.01, ^#p < 0.01, $p < 0.001, ¶ p < 0.0001 Young EVs vs. Aged EVs. D. Representative Β-gal staining of P5 HAEC incubated with the same EVs as in (B) (N = 3 Mean ± SD. *p < 0.001, ^#p < 0.0001 Young EVs vs. Aged EVs). Scale bar = 50 μm