Fig. 2

Proteomic analysis of EVs isolated from young and aged ApoE KO mice. A, B. Functional enrichment analysis of proteins present in blood circulating EVs from aged vs. young atherosclerotic ApoE null mice. C. Differences between young and aged EV samples, as well as the variability within each group, visualized through Principal Component Analysis (PCA). D. The volcano plot represents the quantitative proteomics data of samples, highlighting proteins with significant differential abundance. In this plot, proteins with statistical significance are defined as those with a -log10 p-value > 1.3 (y-axis). Statistically underexpressed proteins (green square) have a log2 fold change ≤ -1, while statistically overexpressed proteins (red square) have a log2 fold change ≥ + 1 (x-axis)