Fig. 2

Expression of Ncoa4 and Adamts3/8/12/14 was dependent on Prmt1 in MC3T3-E1 cells. (A) Schematic of iTRAQ analysis in Control^KD and Prmt1^KD cells (MC3T3-E1 background) treated with PBS or 40 ng/mL IL-1β for 6 h. (B and C) Volcano plots illustrating the log2 fold changes. Red dots represent upregulated proteins; grey dots represent unchanged proteins; blue dots represent downregulated proteins. (B) PBS vs. IL-1β in Control^KD cells. (C) PBS vs. IL-1β in Prmt1^KD cells. (D) Heatmap showing the top 20 most significantly dysregulated proteins. (E and F) Validation of protein expression levels for Prmt1, Ncoa4, Adamts3/8/12/14, Hnrnkp, Habp4, Grwd1, Btg2, Ctu1, G3bp2, and Cyth2 in Control^KD and Prmt1^KD cells treated with PBS and 40 ng/mL IL-1β for 6 h