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Fig. 6 | Biology Direct

Fig. 6

From: Prmt1-mediated methylation regulates Ncoa4 stability to transactivate Adamts genes and promote bone extracellular matrix degradation in chronic hematogenous osteomyelitis

Fig. 6

Knockdown of Ncoa4-Cbp-Ap1 components reduced the expression of Adamts3/8/12/14. (A) Schematic representation of Ap1 transcription factor binding sites on Adamts1/3/4/8/12/14 promoters (2500 bp length). (B) Quantitative analysis of Ncoa4, Cbp, c-Jun, and c-Fos mRNA expression levels in MC3T3-E1 cells following knockdown experiments (ControlKD1/2, Ncoa4KD1/2, CbpKD1/2, c-JunKD1/2, and c-FosKD1/2). (C) Quantitative mRNA expression profiles of Adamts1/3/4/8/12/14 in ControlKD1, Ncoa4KD1, CbpKD1, c-JunKD1, and c-FosKD1 cells treated with or without IL-1β (40 ng/mL). (D) ChIP analysis. Cells from (C) were subjected to ChIP assays using specific antibodies against Ncoa4, Cbp, c-Jun, c-Fos, and IgG-conjugated protein G agarose. Enrichment of Ncoa4-Cbp-Ap1 components on the promoter of Adamts3 was quantified by RT-qPCR analysis of input and immunoprecipitated DNA samples. *P < 0.05; ***P < 0.001

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Biology Direct

ISSN: 1745-6150

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