Fig. 6

BTF3L4 was the common target gene of miR-192 and miR-194 in GC. (A) The common differentially expressed genes of miR-192 and miR-194 were listed in the heat map. (B) RNA-seq analysis showed that BTF3L4 expression was downregulated by either miR-194 or miR-192 in GC cell lines. (C) Bioinformatics prediction showed that the 3’UTR of BTF3L4 contains the binding sites of miR-194 and miR-192. (D) Overexpression of miR-194 or miR-192, or both, significantly decreased the expression level of BTF3L4 in GC cell lines. (E) Western blotting assays showed that the overexpression of miR-194 or miR-192 significantly decreased the expression level of BTF3L4 in GC cell lines. (F-H) Luciferase reporter assays showed that BTF3L4 was directly targeted by miR-194 or miR-192. (I) Overall survival analysis showed that low expression levels of miR-194 or miR-192 predicted a poor prognosis in GC tissues. (J) BTF3L4 expression showed a significant negative correlation with miR-194/192 and MIR194-2HG in GC tissues. **, P < 0.01