Fig. 5

FOXP2-mediated transcriptional repression of CEP55 impedes OC cell malignant phenotype. A, CEP55 expression after overexpression of CEP55 in A2780 and SKOV3 cells overexpressing FOXP2 was analyzed using RT-qPCR. B, Colony formation in A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 was assessed using colony formation assays. C, The proliferative capacity of A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 was evaluated using EdU staining. D, Apoptosis in A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 was evaluated using TUNEL assay. E, Cell cycle changes in A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 were detected by flow cytometry. F-G, The migration and invasion of A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 was detected by Transwell assay. H, Alterations in the cytoskeleton of A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 were assessed using Phalloidin staining. I, Expression of EMT-related proteins E-cadherin, Vimentin, Snail, ZEB1, Slug, and N-cadherin in A2780 and SKOV3 cells overexpressing both FOXP2 and CEP55 analyzed using western blot. Statistical significance was assessed using ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All cell experiments were repeated three times