Fig. 8

Knockdown of ALKBH5 leads to cell cycle arrest and inhibits EMT in OC cells. A, Cell cycle changes in A2780 and SKOV3 cells with ALKBH5 knockdown were detected by flow cytometry. B, Cell cycle-related protein expression in A2780 and SKOV3 cells after knockdown of ALKBH5 was assessed using western blot. C-D, The migration and invasion of A2780 and SKOV3 cells after knockdown of ALKBH5 was detected by Transwell assay. E, Alterations in the cytoskeleton of A2780 and SKOV3 cells after knockdown of ALKBH5 were assessed using Phalloidin staining. F, Expression of EMT-related proteins E-cadherin, Vimentin, Snail, ZEB1, Slug, and N-cadherin in A2780 and SKOV3 cells after knockdown of ALKBH5 was analyzed using western blot. Statistical significance was assessed using ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All cell experiments were repeated three times