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Table 1 Primers used for qPCR assay

From: ALKBH5 activates CEP55 transcription through m6A demethylation in FOXP2 mRNA and expedites cell cycle entry and EMT in ovarian cancer

Gene

Forward sequence (5’-3’)

Reverse sequence (5’-3’)

CEP55

TCGACCGTCAACATGTGCAGCA

GGCTCTGTGATGGCAAACTCATG

KI67

GAAAGAGTGGCAACCTGCCTTC

GCACCAAGTTTTACTACATCTGCC

PCNA

CAAGTAATGTCGATAAAGAGGAGG

GTGTCACCGTTGAAGAGAGTGG

FOXP2

TGGATGACCGAAGCACTGCTCA

TGGGAGATGGTTTGGGCTCTGA

ALKBH5

CCAGCTATGCTTCAGATCGCCT

GGTTCTCTTCCTTGTCCATCTCC

β-actin

CACCATTGGCAATGAGCGGTTC

AGGTCTTTGCGGATGTCCACGT

  1. Note CEP55, centrosomal protein of 55 kDa; PCNA, proliferating cell nuclear antigen; FOXP2, Forkhead box protein P2; ALKBH5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
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Biology Direct

ISSN: 1745-6150

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